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Introductory_exercise


Masterclass: Introductory Exercise.
This Zip file contains the manual and a dataset for a short introductory exercise with LinRegPCR. The download of the program itself is described in the manual


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References

  • Ramakers et al. Assumption-free analysis of quantitative real-time PCR data. Neurosci Letters 339: 62-66, 2003
  • Ruijter et al. Amplification efficiency: linking baseline and bias in the analysis of quantitative PCR data. Nucleic Acids Research 37: e45, 2009
  • Tuomi et al. Bias in the Cq value observed with hydrolysis probe based quantitative PCR can be corrected with the estimated PCR efficiency value. Methods 50: 313-322, 2010
  • Ruijter et al. Evaluation of qPCR curve analysis methods for reliable biomarker discovery: bias, resolution, precision, and implications. Methods 59: 32-46, 2013
  • Ruijter et al. Fluorescent-increase kinetics of different fluorescent reporters used for qPCR depend on monitoring chemistry, targeted sequence, type of DNA input and PCR efficiency. Microchimica Acta 2014
  • Ruijter JM, Ruiz Villalba A, Hellemans J, Untergasser A, van den Hoff MJB. Removal of between-run variation in a multi-plate qPCR experiment. Biomolecular Detection and Quantification 5:10-14, 2015.

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Version History

Workshop version 1.1: Released May 2017.

2017 version of the introductory exercise.
The file contains:

  • Introductory Exercise.pdf
  • Introductory Exercise.xls

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Introductory_exercise (automated content)